Hotspot

psbC-trnS-UGA

LSC · 35950-36176 · 227 bp

Back to Glochidion

Pi 0.0213

MarkerSeek score 59.70

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 227
variable_sites: 7
pi: 0.0213
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	AGCTGCAGCAGGATTTGAAA	AAGAACTATCGGGGGTTCGA	45.0	50.0	58.4	58.1	327-335 bp	1.0	79.5
psbC-trnS-UGA_p2	AGCTGCAGCAGGATTTGAAA	AGAACTATCGGGGGTTCGAA	45.0	50.0	58.4	58.1	326-334 bp	1.0	79.5
psbC-trnS-UGA_p3	AGCTGCAGCAGGATTTGAAA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	58.4	58.2	362-370 bp	1.0	78.9
psbC-trnS-UGA_p4	TAGGTCATTTATGGCACGCG	AAGAACTATCGGGGGTTCGA	50.0	50.0	58.4	58.1	361-369 bp	1.0	78.8
psbC-trnS-UGA_p5	TAGGTCATTTATGGCACGCG	AGAACTATCGGGGGTTCGAA	50.0	50.0	58.4	58.1	360-368 bp	1.0	78.8