Hotspot

atpH-atpI

LSC · 13940-15085 · 1146 bp

Back to Glochidion

Pi 0.00133

MarkerSeek score 61.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1146
variable_sites: 3
pi: 0.00133
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCGGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1263-1280 bp	1.0	79.9
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	58.0	57.9	1216-1233 bp	1.0	79.9
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGG	45.0	55.0	58.0	58.2	1190-1207 bp	1.0	79.6
atpH-atpI_p4	CAGTACCTTGACCAACTCCG	CCGGTCATGTTCCTTGGATT	55.0	50.0	57.9	57.9	1322-1339 bp	1.0	79.5
atpH-atpI_p5	CAGTACCTTGACCAACTCCG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.9	57.9	1275-1292 bp	1.0	79.5