Hotspot

atpA-atpF

LSC · 12700-12754 · 55 bp

Back to Glochidion

Pi 0.0242

MarkerSeek score 60.70

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 55
variable_sites: 2
pi: 0.0242
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	ATTGGCATGTTTGGGACGAT	55.0	45.0	58.1	58.1	206-208 bp	1.0	79.2
atpA-atpF_p2	TACTAATCTCGTCGGCTCGA	ATTGGCATGTTTGGGACGAT	50.0	45.0	57.8	58.1	124-126 bp	1.0	78.9
atpA-atpF_p3	TAATCTCGTCGGCTCGAATG	ATTGGCATGTTTGGGACGAT	50.0	45.0	57.6	58.1	121-123 bp	1.0	77.6
atpA-atpF_p4	CCGTCGCCTACTTGAAGTAC	TATTGGCATGTTTGGGACGA	55.0	45.0	58.1	57.5	207-209 bp	1.0	76.4
atpA-atpF_p5	ACCGTCGCCTACTTGAAGTA	ATTGGCATGTTTGGGACGAT	50.0	45.0	58.5	58.1	207-209 bp	1.0	76.3