Hotspot

trnR-UCU-atpA

LSC · 10899-11175 · 277 bp

Back to Glochidion

Pi 0.0126

MarkerSeek score 50.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 277
variable_sites: 7
pi: 0.0126
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AAGCGTCCATCGTCTAATGG	TCAAGAACAGCTGGAACGTT	50.0	45.0	57.8	57.7	386-415 bp	1.0	78.6
trnR-UCU-atpA_p2	AAGCGTCCATCGTCTAATGG	TTCAAGAACAGCTGGAACGT	50.0	45.0	57.8	57.7	387-416 bp	1.0	78.6
trnR-UCU-atpA_p3	GGAAAAGCGTCCATCGTCTA	TCAAGAACAGCTGGAACGTT	50.0	45.0	57.7	57.7	390-419 bp	1.0	78.1
trnR-UCU-atpA_p4	GGAAAAGCGTCCATCGTCTA	TTCAAGAACAGCTGGAACGT	50.0	45.0	57.7	57.7	391-420 bp	1.0	78.1
trnR-UCU-atpA_p5	AAGCGTCCATCGTCTAATGG	GGAAGCCCTTTTGAAAGAAGC	50.0	47.6	57.8	58.0	410-439 bp	1.0	72.8