Hotspot

trnS-GCU-trnG-UCC

LSC · 8954-9715 · 762 bp

Back to Glochidion

Pi 0.00262

MarkerSeek score 64.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 4
pi: 0.00262
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.6	826-834 bp	1.0	55.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TGAACGAATCACACTTTTACCAC	50.0	39.1	58.0	57.6	825-833 bp	1.0	55.7
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	TGAACCGAGAGATCCTTTAACT	50.0	40.9	58.0	56.6	860-868 bp	1.0	55.5
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	TGAACCGAGAGATCCTTTAACT	50.0	40.9	58.0	56.6	859-867 bp	1.0	55.5
trnS-GCU-trnG-UCC_p5	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	823-831 bp	1.0	54.8