Hotspot

rps19-rpl2

IRb · 84321-84377 · 57 bp

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Pi 0.0351

MarkerSeek score 74.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 57
variable_sites: 3
pi: 0.0351
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	CACGGGACCAAGTTACGATT	GATTGTTCGTCGCCGTAGTA	50.0	50.0	57.9	57.8	191-195 bp	1.0	79.1
rps19-rpl2_p2	CACGGGACCAAGTTACGATT	CACAACCCCTTGGGGTTATC	50.0	55.0	57.9	58.2	260-264 bp	1.0	79.0
rps19-rpl2_p3	CACGGGACCAAGTTACGATT	TTTGATTGTTCGTCGCCGTA	50.0	45.0	57.9	58.2	194-198 bp	1.0	78.8
rps19-rpl2_p4	CACGGGACCAAGTTACGATT	AATTTGATTGTTCGTCGCCG	50.0	45.0	57.9	57.8	196-200 bp	1.0	78.6
rps19-rpl2_p5	CACGGGACCAAGTTACGATT	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.9	57.8	251-255 bp	1.0	78.5