Hotspot

rbcL-accD

LSC · 57310-57962 · 653 bp

Back to Globularia

Pi 0.0214

MarkerSeek score 59.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 21
pi: 0.0214
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGGCTAGCAAATGGAGTCC	CATCGAATTTACCCACCACCT	55.0	47.6	58.0	58.0	797-847 bp	1.0	73.7
rbcL-accD_p2	GCTGCTTGTGAGGTATGGAA	CATCGAATTTACCCACCACCT	50.0	47.6	57.9	58.0	767-817 bp	1.0	73.2
rbcL-accD_p3	GAGTCCTGAACTAGCTGCTG	CATCGAATTTACCCACCACCT	55.0	47.6	57.7	58.0	783-833 bp	1.0	71.7
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	TCGAATTTACCCACCACCTT	55.0	45.0	58.0	56.7	795-845 bp	1.0	71.7
rbcL-accD_p5	GAGGCTAGCAAATGGAGTCC	ACATCGAATTTACCCACCACC	55.0	47.6	58.0	58.3	798-848 bp	1.0	71.5