Hotspot

ndhC-trnV-UAC

LSC · 50929-52033 · 1105 bp

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Pi 0.02

MarkerSeek score 74.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1105
variable_sites: 33
pi: 0.02
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1210-1253 bp	1.0	82.5
ndhC-trnV-UAC_p2	TTCTCCGGCCCTTTCCTAAT	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.4	58.1	1231-1274 bp	1.0	79.8
ndhC-trnV-UAC_p3	CCCTTTCCTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.6	58.1	1223-1266 bp	1.0	78.6
ndhC-trnV-UAC_p4	TTTCTCCGGCCCTTTCCTAA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	58.6	58.1	1232-1275 bp	1.0	78.0
ndhC-trnV-UAC_p5	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1195-1238 bp	1.0	77.9