Hotspot

trnF-GAA-ndhJ

LSC · 48540-49251 · 712 bp

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Pi 0.00976

MarkerSeek score 63.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 10
pi: 0.00976
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	844-855 bp	1.0	83.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	808-819 bp	1.0	82.7
trnF-GAA-ndhJ_p3	TCAAATCTGGTTCCTGGCAC	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	793-804 bp	1.0	82.5
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	832-843 bp	1.0	81.9
trnF-GAA-ndhJ_p5	GGAAATGGTCGGGATAGCTC	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.8	58.1	854-865 bp	1.0	81.6