Hotspot

trnT-UGU-trnL-UAA

LSC · 46653-47563 · 911 bp

Back to Globularia

Pi 0.0129

MarkerSeek score 61.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 911
variable_sites: 16
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	AGAGGCAAAAGCCAGCTATC	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	939-1025 bp	1.0	82.9
trnT-UGU-trnL-UAA_p2	AAAGCCAGCTATCGGAATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.1	58.2	932-1018 bp	1.0	82.3
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	887-973 bp	1.0	82.3
trnT-UGU-trnL-UAA_p4	AGAGGCAAAAGCCAGCTATC	CGTCTACCGATTTCGCCATA	50.0	50.0	58.0	57.6	933-1019 bp	1.0	81.6
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	919-1005 bp	1.0	81.4