Hotspot

rps4-trnT-UGU

LSC · 46206-46577 · 372 bp

Back to Globularia

Pi 0.0287

MarkerSeek score 64.50

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 372
variable_sites: 16
pi: 0.0287
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	57.6	57.9	501-538 bp	1.0	78.2
rps4-trnT-UGU_p2	GTTAGTCCTGGTAAAGCCCC	GGTTCGATTCCGATAGCTGG	55.0	55.0	57.6	58.2	460-497 bp	1.0	77.8
rps4-trnT-UGU_p3	GTTAGTCCTGGTAAAGCCCC	ATGGTCATCGGTTCGATTCC	55.0	50.0	57.6	57.8	469-506 bp	1.0	77.5
rps4-trnT-UGU_p4	TTAGTCCTGGTAAAGCCCCC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.7	57.9	500-537 bp	1.0	75.9
rps4-trnT-UGU_p5	GATCACTTCCGGCTTTAGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.7	57.9	530-567 bp	1.0	75.8