Hotspot

trnG-GCC-trnM-CAU

LSC · 36315-36467 · 153 bp

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Pi 0.0174

MarkerSeek score 63.60

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 4
pi: 0.0174
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	AAAAACGCGGATATGGTCGA	TTACTTCTTCTCTTCGGCGC	45.0	50.0	58.0	58.0	323-335 bp	1.0	80.8
trnG-GCC-trnM-CAU_p2	AAAACGCGGATATGGTCGAA	TTACTTCTTCTCTTCGGCGC	45.0	50.0	58.0	58.0	322-334 bp	1.0	80.8
trnG-GCC-trnM-CAU_p3	AAAACGCGGATATGGTCGAA	CTTGAGGTCACGGGTTCAAA	45.0	50.0	58.0	58.0	265-277 bp	1.0	80.8
trnG-GCC-trnM-CAU_p4	AAAAACGCGGATATGGTCGA	GGGTAGAGCAGTTTGGTAGC	45.0	55.0	58.0	58.0	302-314 bp	1.0	80.7
trnG-GCC-trnM-CAU_p5	AAAACGCGGATATGGTCGAA	GGGTAGAGCAGTTTGGTAGC	45.0	55.0	58.0	58.0	301-313 bp	1.0	80.7