Hotspot

trnS-UGA-psbZ

LSC · 35408-35738 · 331 bp

Back to Globularia

Pi 0.0201

MarkerSeek score 64.40

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 331
variable_sites: 10
pi: 0.0201
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	GAGAAGCAAATACGACGGGT	55.0	50.0	58.0	58.0	440-443 bp	1.0	82.9
trnS-UGA-psbZ_p2	GTTTTCAAGACCGGGGCTAT	GAGAAGCAAATACGACGGGT	50.0	50.0	57.9	58.0	460-463 bp	1.0	81.9
trnS-UGA-psbZ_p3	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	451-454 bp	1.0	81.2
trnS-UGA-psbZ_p4	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	453-456 bp	1.0	80.8
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	AGGAGAAGCAAATACGACGG	55.0	50.0	58.0	57.7	442-445 bp	1.0	80.5