Hotspot

trnE-UUC-trnT-GGU

LSC · 30735-31373 · 639 bp

Back to Globularia

Pi 0.0145

MarkerSeek score 64.00

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 13
pi: 0.0145
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	745-781 bp	1.0	85.1
trnE-UUC-trnT-GGU_p2	CATAGTACCCTACCCCCAGG	TACCGCTGAGTTAAAAGGGC	60.0	50.0	58.0	57.9	710-746 bp	1.0	84.4
trnE-UUC-trnT-GGU_p3	CATAGTACCCTACCCCCAGG	AATGAGAGCCCCTTATCGGA	60.0	50.0	58.0	57.9	768-804 bp	1.0	84.3
trnE-UUC-trnT-GGU_p4	CATAGTACCCTACCCCCAGG	TGAGAGCCCCTTATCGGATT	60.0	50.0	58.0	57.9	766-802 bp	1.0	84.3
trnE-UUC-trnT-GGU_p5	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	60.0	55.0	58.0	58.2	742-778 bp	1.0	83.9