Hotspot

trnG-UCC-trnR-UCU

LSC · 9699-9975 · 277 bp

Back to Globularia

Pi 0.0587

MarkerSeek score 78.60

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 277
variable_sites: 21
pi: 0.0587
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	356-480 bp	1.0	70.7
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.1	56.9	359-483 bp	1.0	70.4
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	362-486 bp	1.0	69.9
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.1	57.9	365-489 bp	1.0	69.6
trnG-UCC-trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	AGCGTTTCTTGCTATCGAAA	50.0	40.0	58.0	55.8	434-558 bp	1.0	68.3