primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC_p1	trnG-UCC	1	AGGGGTCCTTCCTTTTCTGA	TTCAGGAACAAATGGACCGG	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.229571	8950	9698	1799-1943	1.000000	81.1
trnG-UCC_p2	trnG-UCC	2	TGAGTCTGTCCTTCTCTCCC	TTCAGGAACAAATGGACCGG	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.319517	8950	9698	1986-2126	1.000000	80.7
trnG-UCC_p3	trnG-UCC	3	CGCGAACAACCGAACTAAAG	TTCAGGAACAAATGGACCGG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.302754	8950	9698	1861-2011	1.000000	80.6
trnG-UCC_p4	trnG-UCC	4	TTCCTTTTCTGATGGCCTGG	TTCAGGAACAAATGGACCGG	20	20	50.0	50.0	57.8	58.1	3.3	0.0	0.316366	8950	9698	1791-1935	1.000000	80.5
trnG-UCC_p5	trnG-UCC	5	TTTGTTCAAGGGGTCCTTCC	TTCAGGAACAAATGGACCGG	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.470727	8950	9698	1807-1951	1.000000	79.2
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9699	9975	356-480	1.000000	70.7
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9699	9975	359-483	1.000000	70.4
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9699	9975	362-486	1.000000	69.9
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9699	9975	365-489	1.000000	69.6
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	AGCCTTCCAAGCTAACGATG	AGCGTTTCTTGCTATCGAAA	20	20	50.0	40.0	58.0	55.8	0.0	0.0	2.236662	9699	9975	434-558	1.000000	68.3
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCATTTGCATTCCCCAGCAT	CCGGATTTGAACTGGGGAAA	20	20	45.0	50.0	58.1	58.1	0.0	0.0	0.213415	26888	28128	1274-1323	1.000000	82.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTCCCTCATTTGCATTCCCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.217459	26888	28128	1279-1328	1.000000	82.0
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCATTTGCATTCCCCAGCAT	AAAAGGATTTGCAGTCCCCC	20	20	45.0	50.0	58.1	58.4	0.0	14.6	0.486778	26888	28128	1256-1305	1.000000	79.9
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCATTTGCATTCCCC	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.1	58.4	0.0	14.6	0.490822	26888	28128	1261-1310	1.000000	79.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	CTCATTTGCATTCCCCAGCA	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.8	58.1	0.0	0.0	0.894154	26888	28128	1275-1324	1.000000	76.6
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	20	20	60.0	50.0	58.0	58.0	0.0	0.0	0.031606	30735	31373	745-781	1.000000	85.1
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CATAGTACCCTACCCCCAGG	TACCGCTGAGTTAAAAGGGC	20	20	60.0	50.0	58.0	57.9	0.0	0.0	0.129173	30735	31373	710-746	1.000000	84.4
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CATAGTACCCTACCCCCAGG	AATGAGAGCCCCTTATCGGA	20	20	60.0	50.0	58.0	57.9	0.0	0.0	0.128789	30735	31373	768-804	1.000000	84.3
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CATAGTACCCTACCCCCAGG	TGAGAGCCCCTTATCGGATT	20	20	60.0	50.0	58.0	57.9	0.0	0.0	0.128789	30735	31373	766-802	1.000000	84.3
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	20	20	60.0	55.0	58.0	58.2	0.0	0.0	0.176539	30735	31373	742-778	1.000000	83.9
trnS-UGA-psbZ_p1	trnS-UGA-psbZ	1	CAACCACTCAGCCATCTCTC	GAGAAGCAAATACGACGGGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.027326	35408	35738	440-443	1.000000	82.9
trnS-UGA-psbZ_p2	trnS-UGA-psbZ	2	GTTTTCAAGACCGGGGCTAT	GAGAAGCAAATACGACGGGT	20	20	50.0	50.0	57.9	58.0	0.8	0.0	0.130248	35408	35738	460-463	1.000000	81.9
trnS-UGA-psbZ_p3	trnS-UGA-psbZ	3	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.226377	35408	35738	451-454	1.000000	81.2
trnS-UGA-psbZ_p4	trnS-UGA-psbZ	4	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	20	20	55.0	55.0	58.0	58.3	0.0	0.0	0.277359	35408	35738	453-456	1.000000	80.8
trnS-UGA-psbZ_p5	trnS-UGA-psbZ	5	CAACCACTCAGCCATCTCTC	AGGAGAAGCAAATACGACGG	20	20	55.0	50.0	58.0	57.7	0.0	0.0	0.316608	35408	35738	442-445	1.000000	80.5
trnG-GCC-trnM-CAU_p1	trnG-GCC-trnM-CAU	1	AAAAACGCGGATATGGTCGA	TTACTTCTTCTCTTCGGCGC	20	20	45.0	50.0	58.0	58.0	12.5	0.0	0.020767	36315	36467	323-335	1.000000	80.8
trnG-GCC-trnM-CAU_p2	trnG-GCC-trnM-CAU	2	AAAACGCGGATATGGTCGAA	TTACTTCTTCTCTTCGGCGC	20	20	45.0	50.0	58.0	58.0	12.5	0.0	0.020767	36315	36467	322-334	1.000000	80.8
trnG-GCC-trnM-CAU_p3	trnG-GCC-trnM-CAU	3	AAAACGCGGATATGGTCGAA	CTTGAGGTCACGGGTTCAAA	20	20	45.0	50.0	58.0	58.0	12.5	4.2	0.051143	36315	36467	265-277	1.000000	80.8
trnG-GCC-trnM-CAU_p4	trnG-GCC-trnM-CAU	4	AAAAACGCGGATATGGTCGA	GGGTAGAGCAGTTTGGTAGC	20	20	45.0	55.0	58.0	58.0	12.5	0.0	0.038525	36315	36467	302-314	1.000000	80.7
trnG-GCC-trnM-CAU_p5	trnG-GCC-trnM-CAU	5	AAAACGCGGATATGGTCGAA	GGGTAGAGCAGTTTGGTAGC	20	20	45.0	55.0	58.0	58.0	12.5	0.0	0.038525	36315	36467	301-313	1.000000	80.7
psaA-ycf3_p1	psaA-ycf3	1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	20	20	50.0	50.0	57.9	58.0	4.1	0.0	0.156301	41605	42376	931-950	1.000000	81.6
psaA-ycf3_p2	psaA-ycf3	2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	20	20	50.0	50.0	57.9	58.0	4.1	18.7	0.160416	41605	42376	913-932	1.000000	81.6
psaA-ycf3_p3	psaA-ycf3	3	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	4.1	0.0	0.279161	41605	42376	860-879	1.000000	80.8
psaA-ycf3_p4	psaA-ycf3	4	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	57.9	58.2	4.1	0.0	0.306932	41605	42376	864-883	1.000000	80.6
psaA-ycf3_p5	psaA-ycf3	5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	20	20	50.0	50.0	57.9	58.3	4.1	0.0	0.376276	41605	42376	927-946	1.000000	79.8
rps4-trnT-UGU_p1	rps4-trnT-UGU	1	GTTAGTCCTGGTAAAGCCCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	57.6	57.9	0.0	0.0	0.547734	46206	46577	501-538	1.000000	78.2
rps4-trnT-UGU_p2	rps4-trnT-UGU	2	GTTAGTCCTGGTAAAGCCCC	GGTTCGATTCCGATAGCTGG	20	20	55.0	55.0	57.6	58.2	0.0	0.0	0.630468	46206	46577	460-497	1.000000	77.8
rps4-trnT-UGU_p3	rps4-trnT-UGU	3	GTTAGTCCTGGTAAAGCCCC	ATGGTCATCGGTTCGATTCC	20	20	55.0	50.0	57.6	57.8	0.0	0.0	0.655487	46206	46577	469-506	1.000000	77.5
rps4-trnT-UGU_p4	rps4-trnT-UGU	4	TTAGTCCTGGTAAAGCCCCC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.7	57.9	0.0	0.0	0.840655	46206	46577	500-537	1.000000	75.9
rps4-trnT-UGU_p5	rps4-trnT-UGU	5	GATCACTTCCGGCTTTAGGC	CTCAGAGGTTAGAGCATCGC	20	20	55.0	55.0	58.7	57.9	0.0	0.0	0.832259	46206	46577	530-567	1.000000	75.8
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AGAGGCAAAAGCCAGCTATC	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	9.4	10.2	0.298337	46653	47563	939-1025	1.000000	82.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	AAAGCCAGCTATCGGAATCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.1	58.2	0.0	10.2	0.377368	46653	47563	932-1018	1.000000	82.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46653	47563	887-973	1.000000	82.3
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	AGAGGCAAAAGCCAGCTATC	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	58.0	57.6	9.4	0.0	0.460674	46653	47563	933-1019	1.000000	81.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	46653	47563	919-1005	1.000000	81.4
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	58.0	58.1	10.3	0.0	0.089643	48540	49251	844-855	1.000000	83.0
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.139034	48540	49251	808-819	1.000000	82.7
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	TCAAATCTGGTTCCTGGCAC	AGTTGGATAGGATGGCCCTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.177438	48540	49251	793-804	1.000000	82.5
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	57.9	58.1	2.5	0.0	0.223436	48540	49251	832-843	1.000000	81.9
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	GGAAATGGTCGGGATAGCTC	AGTTGGATAGGATGGCCCTT	20	20	55.0	50.0	57.8	58.1	0.0	0.0	0.258479	48540	49251	854-865	1.000000	81.6
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	GGGGCTAAAATCCCGGAAAT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	57.9	58.1	0.7	0.0	0.189672	50929	52033	1210-1253	1.000000	82.5
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TTCTCCGGCCCTTTCCTAAT	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.4	58.1	0.0	0.0	0.515764	50929	52033	1231-1274	1.000000	79.8
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CCCTTTCCTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	20	20	55.0	55.0	58.6	58.1	0.0	0.0	0.676540	50929	52033	1223-1266	1.000000	78.6
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TTTCTCCGGCCCTTTCCTAA	CCGAGAAAGTCTACGGTTCG	20	20	50.0	55.0	58.6	58.1	0.0	0.0	0.743263	50929	52033	1232-1275	1.000000	78.0
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.9	58.7	0.7	0.0	0.765489	50929	52033	1195-1238	1.000000	77.9
rbcL-accD_p1	rbcL-accD	1	GAGGCTAGCAAATGGAGTCC	CATCGAATTTACCCACCACCT	20	21	55.0	47.6	58.0	58.0	18.2	0.0	1.040252	57310	57962	797-847	1.000000	73.7
rbcL-accD_p2	rbcL-accD	2	GCTGCTTGTGAGGTATGGAA	CATCGAATTTACCCACCACCT	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.114529	57310	57962	767-817	1.000000	73.2
rbcL-accD_p3	rbcL-accD	3	GAGTCCTGAACTAGCTGCTG	CATCGAATTTACCCACCACCT	20	21	55.0	47.6	57.7	58.0	13.4	0.0	1.292772	57310	57962	783-833	1.000000	71.7
rbcL-accD_p4	rbcL-accD	4	GAGGCTAGCAAATGGAGTCC	TCGAATTTACCCACCACCTT	20	20	55.0	45.0	58.0	56.7	18.2	0.0	1.297021	57310	57962	795-845	1.000000	71.7
rbcL-accD_p5	rbcL-accD	5	GAGGCTAGCAAATGGAGTCC	ACATCGAATTTACCCACCACC	20	21	55.0	47.6	58.0	58.3	18.2	0.0	1.321020	57310	57962	798-848	1.000000	71.5
accD-psaI_p1	accD-psaI	1	CCAGAAGGGCTTATTCGACC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	0.0	26.9	0.072076	59445	60217	935-955	1.000000	85.8
accD-psaI_p2	accD-psaI	2	CGGCTAGTTTTGGCATGTTG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.0	58.0	0.0	26.9	0.045750	59445	60217	1071-1091	1.000000	85.2
accD-psaI_p3	accD-psaI	3	CCAGAAGGGCTTATTCGACC	TTTGTTTTCGAGACCCCGTT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.086324	59445	60217	1453-1472	1.000000	84.0
accD-psaI_p4	accD-psaI	4	CGGCTAGTTTTGGCATGTTG	TTTGTTTTCGAGACCCCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.059997	59445	60217	1589-1608	1.000000	83.8
accD-psaI_p5	accD-psaI	5	CTTTCGATCGATCCCGGTAC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	20.2	26.9	0.074682	59445	60217	1510-1530	1.000000	83.5
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	58.0	58.5	4.9	0.0	0.574654	69229	70001	874-881	1.000000	78.0
rpl20-rps12_p2	rpl20-rps12	2	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	69229	70001	929-936	1.000000	77.2
rpl20-rps12_p3	rpl20-rps12	3	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	20	20	50.0	50.0	57.9	58.5	0.0	0.0	0.654410	69229	70001	928-935	1.000000	77.2
rpl20-rps12_p4	rpl20-rps12	4	TCCGAGCTATATATCCGCGT	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	58.0	58.7	4.9	9.8	0.764938	69229	70001	837-844	1.000000	76.5
rpl20-rps12_p5	rpl20-rps12	5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	20	20	50.0	55.0	57.9	58.7	0.0	9.8	0.844694	69229	70001	891-898	1.000000	75.7
rps19-rpl2_p1	rps19-rpl2	1	CACGGGACCAAGTTACGATT	GATTGTTCGTCGCCGTAGTA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.267424	84321	84377	191-195	1.000000	79.1
rps19-rpl2_p2	rps19-rpl2	2	CACGGGACCAAGTTACGATT	CACAACCCCTTGGGGTTATC	20	20	50.0	55.0	57.9	58.2	0.0	35.0	0.241621	84321	84377	260-264	1.000000	79.0
rps19-rpl2_p3	rps19-rpl2	3	CACGGGACCAAGTTACGATT	TTTGATTGTTCGTCGCCGTA	20	20	50.0	45.0	57.9	58.2	0.0	0.0	0.308144	84321	84377	194-198	1.000000	78.8
rps19-rpl2_p4	rps19-rpl2	4	CACGGGACCAAGTTACGATT	AATTTGATTGTTCGTCGCCG	20	20	50.0	45.0	57.9	57.8	0.0	0.0	0.330209	84321	84377	196-200	1.000000	78.6
rps19-rpl2_p5	rps19-rpl2	5	CACGGGACCAAGTTACGATT	TTGGGGTTATCCTGCACTTG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.303326	84321	84377	251-255	1.000000	78.5
ndhA_p1	ndhA	1	ACTCGTTTCTTTCTCTTGTTTGAG	TGGGAGAAGTCGATCGTTGA	24	20	37.5	50.0	57.6	58.5	0.0	15.9	4.809948	118527	120684	2230-2241	1.000000	43.3
ndhA_p2	ndhA	2	ACTCGTTTCTTTCTCTTGTTTGA	TGGGAGAAGTCGATCGTTGA	23	20	34.8	50.0	56.6	58.5	0.0	15.9	4.843197	118527	120684	2230-2241	1.000000	43.1
ndhA_p3	ndhA	3	ACTCGTTTCTTTCTCTTGTTTGAG	ATGGGAGAAGTCGATCGTTG	24	20	37.5	50.0	57.6	57.4	0.0	15.9	4.937585	118527	120684	2231-2242	1.000000	42.3
ndhA_p4	ndhA	4	ACTCGTTTCTTTCTCTTGTTTGA	ATGGGAGAAGTCGATCGTTG	23	20	34.8	50.0	56.6	57.4	0.0	15.9	4.970834	118527	120684	2231-2242	1.000000	42.1
ndhA_p5	ndhA	5	ACTCGTTTCTTTCTCTTGTTTGAG	GGGAGAAGTCGATCGTTGAA	24	20	37.5	50.0	57.6	57.4	0.0	15.9	5.000658	118527	120684	2229-2240	1.000000	41.8
ycf1_p1	ycf1	1	TTCCTTAAAAACGGGTTTAAAA	TCTGTATGAAACCTTGGCACA	22	21	27.3	42.9	52.7	57.5	0.0	0.0	8.820742	125369	125488	173-173	1.000000	43.2
ycf1_p2	ycf1	2	TTTCCTTAAAAACGGGTTTAAA	TCTGTATGAAACCTTGGCACA	22	21	27.3	42.9	52.7	57.5	0.0	0.0	8.820742	125369	125488	174-174	1.000000	43.2
ycf1_p3	ycf1	3	TTTCCTTAAAAACGGGTTTAAAA	TCTGTATGAAACCTTGGCACA	23	21	26.1	42.9	53.5	57.5	0.0	0.0	8.966636	125369	125488	174-174	1.000000	43.2
ycf1_p4	ycf1	4	ATTTCCTTAAAAACGGGTTTAA	TCTGTATGAAACCTTGGCACA	22	21	27.3	42.9	52.5	57.5	0.0	0.0	9.046924	125369	125488	175-175	1.000000	43.2
ycf1_p5	ycf1	5	ATTTCCTTAAAAACGGGTTTAAA	TCTGTATGAAACCTTGGCACA	23	21	26.1	42.9	53.3	57.5	0.0	0.0	9.180031	125369	125488	175-175	1.000000	43.2