Hotspot

ycf1

SSC · 130662-136055 · 5394 bp

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Pi 0.0134
MarkerSeek score 45.50
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5394
variable_sites
191
pi
0.0134
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TCCTTGCCTTTCTTGGATCG ACATATCGGTGCCAAACTTGA 50.0 42.9 57.9 57.9 528 bp 0.142857 36.3
ycf1_p2 CCTTGCCTTTCTTGGATCGT ACATATCGGTGCCAAACTTGA 50.0 42.9 58.2 57.9 527 bp 0.142857 35.8
ycf1_p3 TCCTTGCCTTTCTTGGATCG TCGGTGCCAAACTTGAAAAA 50.0 40.0 57.9 56.7 523 bp 0.142857 34.2
ycf1_p4 CCTTGCCTTTCTTGGATCGT TCGGTGCCAAACTTGAAAAA 50.0 40.0 58.2 56.7 522 bp 0.142857 33.7
ycf1_p5 TGGTGCTTACTCTTTTCGCT ACATATCGGTGCCAAACTTGA 45.0 42.9 57.5 57.9 858 bp 0.142857 32.8