Hotspot

ndhF

SSC · 116544-118763 · 2220 bp

Back to Globba

Pi 0.00953
MarkerSeek score 44.90
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2220
variable_sites
48
pi
0.00953
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 CGCGGTACCCATCCTTTTAT AGAGTACCATACCCGTAATTGA 50.0 40.9 57.8 56.1 2866-2929 bp 1.0 51.1
ndhF_p2 CAATATCGCGGTACCCATCC AGAGTACCATACCCGTAATTGA 55.0 40.9 58.3 56.1 2872-2935 bp 1.0 50.9
ndhF_p3 ATATCGCGGTACCCATCCTT AGAGTACCATACCCGTAATTGA 50.0 40.9 58.4 56.1 2870-2933 bp 1.0 50.3
ndhF_p4 AATATCGCGGTACCCATCCT AGAGTACCATACCCGTAATTGA 50.0 40.9 58.4 56.1 2871-2934 bp 1.0 50.2
ndhF_p5 CTTCAATATCGCGGTACCCA AGAGTACCATACCCGTAATTGA 50.0 40.9 57.5 56.1 2875-2938 bp 1.0 49.0