Hotspot

rps15-ycf1

SSC · 130194-130661 · 468 bp

Back to Globba

Pi 0.0497
MarkerSeek score 79.30
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
468
variable_sites
49
pi
0.0497
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.92
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 AACACCATGCATTGATGGGT TTCTTTGGCCCAATTACCGA 45.0 45.0 58.1 57.4 1010-1044 bp 1.0 84.3
rps15-ycf1_p2 AAATAGGCCAGCATACGTCG AGCGAAAAGAGTAAGCACCA 50.0 45.0 58.1 57.5 1088-1124 bp 0.285714 53.6
rps15-ycf1_p3 AACACCATGCATTGATGGGT AGCGAAAAGAGTAAGCACCA 45.0 45.0 58.1 57.5 1338-1374 bp 0.285714 53.2
rps15-ycf1_p4 TCCGCCATAAAAGGTCCAAG AGCGAAAAGAGTAAGCACCA 50.0 45.0 57.9 57.5 1634-1670 bp 0.285714 51.7
rps15-ycf1_p5 AACACCATGCATTGATGGGT TGCTTGTGGATGGGAATCAA 45.0 45.0 58.1 57.4 1277 bp 0.142857 40.4