Hotspot

rpl32-trnL-UAG

SSC · 119637-120513 · 877 bp

Back to Globba

Pi 0.024
MarkerSeek score 65.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
877
variable_sites
45
pi
0.024
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.93
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACATACCCCGAAAAACGTCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.5 57.9 876-946 bp 1.0 82.6
rpl32-trnL-UAG_p2 TGACATACCCCGAAAAACGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.5 57.9 878-948 bp 1.0 82.6
rpl32-trnL-UAG_p3 ACATACCCCGAAAAACGTCA AGCAGCGTGTCTACCAATTT 45.0 45.0 57.5 57.8 869-939 bp 1.0 82.2
rpl32-trnL-UAG_p4 TGACATACCCCGAAAAACGT AGCAGCGTGTCTACCAATTT 45.0 45.0 57.5 57.8 871-941 bp 1.0 82.2
rpl32-trnL-UAG_p5 ACCCCGAAAAACGTCAAGTA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.4 57.9 872-942 bp 1.0 82.1