Hotspot

accD-psaI

LSC · 61400-61824 · 425 bp

Pi 0.0304

MarkerSeek score 78.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 34
pi: 0.0304
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCGACCCAATAGTACCACGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	593-730 bp	1.0	86.5
accD-psaI_p2	TCGACCCAATAGTACCACGT	AGAAACCATTGCAATTGCCG	50.0	45.0	58.2	57.9	598-735 bp	1.0	86.0
accD-psaI_p3	TTCGACCCAATAGTACCACG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.1	58.0	594-731 bp	1.0	80.2
accD-psaI_p4	TCGACCCAATAGTACCACGTA	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.0	58.0	593-730 bp	1.0	79.8
accD-psaI_p5	TTCGACCCAATAGTACCACG	AGAAACCATTGCAATTGCCG	50.0	45.0	57.1	57.9	599-736 bp	1.0	79.7