Hotspot

trnE-UUC-trnT-GGU

LSC · 32253-33098 · 846 bp

Back to Globba

Pi 0.0274
MarkerSeek score 71.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
846
variable_sites
57
pi
0.0274
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 TCCTTTCGTAGTACCCCCAG TCTACCACTGAGTTAAAAGGGC 55.0 45.5 58.1 57.7 925-976 bp 1.0 69.4
trnE-UUC-trnT-GGU_p2 GCTGCCTCCTTGAAAGAGAG TCTACCACTGAGTTAAAAGGGC 55.0 45.5 58.3 57.7 889-940 bp 1.0 68.6
trnE-UUC-trnT-GGU_p3 TGTCCTGAACCACTAGACGA TCTACCACTGAGTTAAAAGGGC 50.0 45.5 57.7 57.7 868-919 bp 1.0 68.6
trnE-UUC-trnT-GGU_p4 TTCCTTTCGTAGTACCCCCA TCTACCACTGAGTTAAAAGGGC 50.0 45.5 57.7 57.7 926-977 bp 1.0 67.9
trnE-UUC-trnT-GGU_p5 AACCACTAGACGATAGGGGC TCTACCACTGAGTTAAAAGGGC 55.0 45.5 58.6 57.7 861-912 bp 1.0 66.2