Hotspot

rpoB-trnC-GCA

LSC · 27423-28006 · 584 bp

Back to Globba

Pi 0.0252
MarkerSeek score 62.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
584
variable_sites
39
pi
0.0252
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AACCCATAGCCGACGATAGA CGACCTCTGATTTTCGTCGT 50.0 50.0 58.0 58.0 1468-2106 bp 1.0 82.9
rpoB-trnC-GCA_p2 ACCCATAGCCGACGATAGAA CGACCTCTGATTTTCGTCGT 50.0 50.0 58.0 58.0 1467-2105 bp 1.0 82.9
rpoB-trnC-GCA_p3 AACCCATAGCCGACGATAGA TCAGAGACCCTCGTGAGTTT 50.0 50.0 58.0 58.0 1702-2329 bp 0.571429 63.8
rpoB-trnC-GCA_p4 ACCCATAGCCGACGATAGAA TCAGAGACCCTCGTGAGTTT 50.0 50.0 58.0 58.0 1701-2328 bp 0.571429 63.8
rpoB-trnC-GCA_p5 CCGCCTACACAAGCAAATTG TCAGAGACCCTCGTGAGTTT 50.0 50.0 58.0 58.0 1847-2474 bp 0.571429 63.7