Hotspot

trnS-GCU-trnG-GCC

LSC · 8452-9131 · 680 bp

Back to Globba

Pi 0.0279
MarkerSeek score 68.10
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
680
variable_sites
42
pi
0.0279
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA CCGAGATACCCCTTCCCTTA 50.0 55.0 58.0 57.6 779-850 bp 1.0 85.0
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC CCGAGATACCCCTTCCCTTA 50.0 55.0 58.0 57.6 778-849 bp 1.0 85.0
trnS-GCU-trnG-GCC_p3 GAGCGGAGAAAGAGGGATTC CCGAGATACCCCTTCCCTTA 55.0 55.0 57.8 57.6 835-906 bp 1.0 82.9
trnS-GCU-trnG-GCC_p4 AGCAATCCGACGCTTTAGTC CCGAGATACCCCTTCCCTTA 50.0 55.0 58.4 57.6 781-852 bp 1.0 82.2
trnS-GCU-trnG-GCC_p5 CGACGCTTTAGTCCACTCAG CCGAGATACCCCTTCCCTTA 55.0 55.0 58.4 57.6 774-845 bp 1.0 82.0