Hotspot

trnK-UUU-rps16

LSC · 4206-5013 · 808 bp

Back to Globba

Pi 0.0271
MarkerSeek score 61.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
808
variable_sites
51
pi
0.0271
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.91
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TGAGGATCAGTCGTGGTCTT AGGATTCCCCTGGGACTATG 50.0 55.0 58.1 57.9 1245-1290 bp 1.0 86.2
trnK-UUU-rps16_p2 AATCAATGGTCTCCGAACGG AGGATTCCCCTGGGACTATG 50.0 55.0 58.0 57.9 1654 bp 0.142857 44.5
trnK-UUU-rps16_p3 AATCAATGGTCTCCGAACGG GGATTCCCCTGGGACTATGT 50.0 55.0 58.0 58.2 1653 bp 0.142857 44.1
trnK-UUU-rps16_p4 AATCAATGGTCTCCGAACGG CGTTTCTGGGGGAGATTGTT 50.0 50.0 58.0 57.8 1548 bp 0.142857 43.9
trnK-UUU-rps16_p5 AATCAATGGTCTCCGAACGG TGCTGCCCTTTTGAAAAGGA 50.0 45.0 58.0 58.2 1840 bp 0.142857 43.8