Hotspot

ndhG-ndhI

SSC · 128434-128967 · 534 bp

Back to Gleditsia

Pi 0.106

MarkerSeek score 80.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 534
variable_sites: 98
pi: 0.106
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AAGACCTGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	45.0	45.0	55.4	57.9	565-666 bp	1.0	70.7
ndhG-ndhI_p2	AAGACCTGATCCCAGAAAGA	TTTGGGCCGTTTACCAATGT	45.0	45.0	55.4	58.3	554-655 bp	1.0	69.2
ndhG-ndhI_p3	AGACCTGATCCCAGAAAGAT	AATCAAATTGCTTTGGGCCG	45.0	45.0	55.2	57.9	564-665 bp	1.0	68.8
ndhG-ndhI_p4	AAGACCTGATCCCAGAAAGA	CTTTGGGCCGTTTACCAATG	45.0	50.0	55.4	57.6	555-656 bp	1.0	68.6
ndhG-ndhI_p5	AAGACCTGATCCCAGAAAGA	CAAATTGCTTTGGGCCGTTT	45.0	45.0	55.4	58.4	562-663 bp	1.0	68.3