Hotspot

rpl32-trnL-UAG

SSC · 122940-123744 · 805 bp

Back to Gleditsia

Pi 0.024

MarkerSeek score 72.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 805
variable_sites: 40
pi: 0.024
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCATTGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	897-1013 bp	1.0	87.3
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCATTGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	898-1014 bp	1.0	87.3
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCATTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	898-1014 bp	1.0	87.3
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCATTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	899-1015 bp	1.0	87.2
rpl32-trnL-UAG_p5	GGGATATTGGGCAGCATTGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	907-1023 bp	1.0	86.3