Hotspot

ndhF-rpl32

SSC · 121811-122771 · 961 bp

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Pi 0.0135

MarkerSeek score 70.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 961
variable_sites: 28
pi: 0.0135
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCGTGGGAAAAAGGAGAAGT	TCAATGCTGCCCAATATCCC	50.0	50.0	57.7	58.0	1133-1169 bp	1.0	82.9
ndhF-rpl32_p2	CCGTGGGAAAAAGGAGAAGT	CAATGCTGCCCAATATCCCT	50.0	50.0	57.7	58.0	1132-1168 bp	1.0	82.9
ndhF-rpl32_p3	TTTGTTGCCGTGGGAAAAAG	TCAATGCTGCCCAATATCCC	45.0	50.0	57.7	58.0	1140-1176 bp	1.0	82.6
ndhF-rpl32_p4	TTTGTTGCCGTGGGAAAAAG	CAATGCTGCCCAATATCCCT	45.0	50.0	57.7	58.0	1139-1175 bp	1.0	82.6
ndhF-rpl32_p5	CCGTGGGAAAAAGGAGAAGT	AAAAGCTTTCAATGCTGCCC	50.0	45.0	57.7	57.8	1141-1177 bp	1.0	81.5