Hotspot

rps3-rps19

LSC · 92786-93414 · 629 bp

Back to Gleditsia

Pi 0.0354

MarkerSeek score 81.90

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 629
variable_sites: 44
pi: 0.0354
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	TACCAAGTCTGAAACCGAGC	ACTCTAAACTTCCGGGGACA	50.0	50.0	57.5	58.0	631-811 bp	1.0	85.1
rps3-rps19_p2	GTCTGAAACCGAGCGGATTT	ACTCTAAACTTCCGGGGACA	50.0	50.0	58.6	58.0	625-805 bp	1.0	84.3
rps3-rps19_p3	TGGTTGTGCAAACCAAAGAG	ACTCTAAACTTCCGGGGACA	45.0	50.0	57.0	58.0	669-849 bp	1.0	80.8
rps3-rps19_p4	TCTGAAACCGAGCGGATTTA	ACTCTAAACTTCCGGGGACA	45.0	50.0	57.0	58.0	624-804 bp	1.0	80.4
rps3-rps19_p5	TACCAAGTCTGAAACCGAGC	CTAAACTTCCGGGGACATGC	50.0	55.0	57.5	58.6	628-808 bp	1.0	80.3