Hotspot

rps8-rpl14

LSC · 89491-89958 · 468 bp

Back to Gleditsia

Pi 0.0747

MarkerSeek score 93.10

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 72
pi: 0.0747
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 93.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCAATGGTGTCTTTACCCA	TCGAATTTTTGGTGCGATCG	45.0	45.0	57.0	57.5	526-973 bp	1.0	80.1
rps8-rpl14_p2	AGCAATGGTGTCTTTACCCA	GAATTTTTGGTGCGATCGCC	45.0	50.0	57.0	58.7	524-971 bp	1.0	78.4
rps8-rpl14_p3	TCAGCAATGGTGTCTTTACCC	TCGAATTTTTGGTGCGATCG	47.6	45.0	58.2	57.5	528-975 bp	1.0	78.2
rps8-rpl14_p4	CAGCAATGGTGTCTTTACCC	TCGAATTTTTGGTGCGATCG	50.0	45.0	56.7	57.5	527-974 bp	1.0	77.4
rps8-rpl14_p5	TCAGCAATGGTGTCTTTACCC	GAATTTTTGGTGCGATCGCC	47.6	50.0	58.2	58.7	526-973 bp	1.0	76.5