Hotspot

rps11-rpl36

LSC · 88087-88483 · 397 bp

Back to Gleditsia

Pi 0.0914

MarkerSeek score 91.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 397
variable_sites: 76
pi: 0.0914
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 91.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TACGCGCATTCTTACGTGAA	CGTAGGCGTGGACGAATTAG	45.0	55.0	58.0	58.5	487-592 bp	1.0	87.6
rps11-rpl36_p2	TTACGCGCATTCTTACGTGA	CGTAGGCGTGGACGAATTAG	45.0	55.0	58.0	58.5	488-593 bp	1.0	87.6
rps11-rpl36_p3	CCCTTTGGTATTTTACGCGC	CGTAGGCGTGGACGAATTAG	50.0	55.0	57.8	58.5	500-605 bp	1.0	86.3
rps11-rpl36_p4	TACGCGCATTCTTACGTGAA	AGGCGTGGACGAATTAGAGT	45.0	50.0	58.0	58.8	484-589 bp	1.0	85.3
rps11-rpl36_p5	TTACGCGCATTCTTACGTGA	AGGCGTGGACGAATTAGAGT	45.0	50.0	58.0	58.8	485-590 bp	1.0	85.3