Hotspot

ndhC-trnV-UAC

LSC · 57805-58785 · 981 bp

Back to Gleditsia

Pi 0.0996
MarkerSeek score 79.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
981
variable_sites
154
pi
0.0996
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.74
markerseek_score
79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 692-1124 bp 1.0 87.7
ndhC-trnV-UAC_p2 CCTTTGCTAATCGGGGCTAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 691-1123 bp 1.0 84.9
ndhC-trnV-UAC_p3 CTCTGGCCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 699-1131 bp 1.0 84.8
ndhC-trnV-UAC_p4 TCTCTGGCCCTTTGCTAATC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 700-1132 bp 1.0 84.7
ndhC-trnV-UAC_p5 CCTTTGCTAATCGGGGCTAA GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 680-1112 bp 1.0 83.8