Hotspot

psbZ-trnG-GCC

LSC · 41493-42512 · 1020 bp

Back to Gleditsia

Pi 0.0983

MarkerSeek score 85.00

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 1020
variable_sites: 193
pi: 0.0983
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CCGGTACATCATTATGGATTGGA	GCGTCTTCTCTTTGGCAAAG	43.5	50.0	58.1	57.7	679-1602 bp	1.0	64.7
psbZ-trnG-GCC_p2	TCCGGTACATCATTATGGATTGG	GCGTCTTCTCTTTGGCAAAG	43.5	50.0	58.1	57.7	680-1603 bp	1.0	64.7
psbZ-trnG-GCC_p3	CCGGTACATCATTATGGATTGG	GCGTCTTCTCTTTGGCAAAG	45.5	50.0	56.7	57.7	679-1602 bp	1.0	63.0
psbZ-trnG-GCC_p4	CCGGTACATCATTATGGATTGGA	AACCCGCGTCTTCTCTTTG	43.5	52.6	58.1	58.1	684-1607 bp	1.0	58.6
psbZ-trnG-GCC_p5	TCCGGTACATCATTATGGATTGG	AACCCGCGTCTTCTCTTTG	43.5	52.6	58.1	58.1	685-1608 bp	1.0	58.6