Hotspot

trnR-UCU-atpA

LSC · 12208-12756 · 549 bp

Back to Gleditsia

Pi 0.0988

MarkerSeek score 80.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 549
variable_sites: 91
pi: 0.0988
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	GGAACTCTTTTTACTTCAGGAACA	47.6	37.5	57.3	57.1	483-1180 bp	1.0	52.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TGGAACTCTTTTTACTTCAGGAAC	47.6	37.5	57.3	57.1	484-1181 bp	1.0	52.0
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TGGAACTCTTTTTACTTCAGGA	47.6	36.4	57.3	55.0	484-1181 bp	1.0	52.0
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	TGGAACTCTTTTTACTTCAGGAA	47.6	34.8	57.3	55.7	484-1181 bp	1.0	52.0
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	GGAACTCTTTTTACTTCAGGAACAA	47.6	36.0	57.3	57.7	483-1180 bp	1.0	52.0