primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.0	58.1	0.0	16.9	0.081289	6717	8383	817-1926	0.833333	84.7
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	0.0	16.9	0.210236	6717	8383	819-1928	0.833333	83.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.8	58.1	0.0	16.9	0.272396	6717	8383	805-1914	0.833333	83.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.8	58.1	0.0	16.9	0.293454	6717	8383	820-1929	0.833333	83.0
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TGTCTTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.4	58.1	0.0	16.9	0.428755	6717	8383	818-1927	0.833333	81.9
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	CTAACGATGCGGGTTCGATT	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.4	52.4	0.0	0.0	7.001259	11694	12135	499-525	1.000000	50.0
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	AGCCTTCCAAGCTAACGATG	TGTCCTATCCATTAGACAATG	20	21	50.0	38.1	58.0	52.4	0.0	0.0	6.598602	11694	12135	510-536	1.000000	49.8
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	CCTAGCCTTCCAAGCTAACG	TGTCCTATCCATTAGACAATG	20	21	55.0	38.1	58.1	52.4	0.0	0.0	6.627438	11694	12135	513-539	1.000000	49.8
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCCTAGCCTTCCAAGCTAAC	TGTCCTATCCATTAGACAATG	20	21	55.0	38.1	57.7	52.4	0.0	0.0	6.902452	11694	12135	514-540	1.000000	49.8
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	ACCAAAGTTTCACGTTCGGT	TGTCCTATCCATTAGACAATG	20	21	45.0	38.1	58.3	52.4	0.0	0.0	6.827235	11694	12135	580-606	1.000000	49.2
trnR-UCU-atpA_p1	trnR-UCU-atpA	1	GGTTCAAATCCTATTGGACGC	GGAACTCTTTTTACTTCAGGAACA	21	24	47.6	37.5	57.3	57.1	0.0	0.0	6.630227	12208	12756	483-1180	1.000000	52.0
trnR-UCU-atpA_p2	trnR-UCU-atpA	2	GGTTCAAATCCTATTGGACGC	TGGAACTCTTTTTACTTCAGGAAC	21	24	47.6	37.5	57.3	57.1	0.0	0.0	6.630227	12208	12756	484-1181	1.000000	52.0
trnR-UCU-atpA_p3	trnR-UCU-atpA	3	GGTTCAAATCCTATTGGACGC	TGGAACTCTTTTTACTTCAGGA	21	22	47.6	36.4	57.3	55.0	0.0	0.0	6.742320	12208	12756	484-1181	1.000000	52.0
trnR-UCU-atpA_p4	trnR-UCU-atpA	4	GGTTCAAATCCTATTGGACGC	TGGAACTCTTTTTACTTCAGGAA	21	23	47.6	34.8	57.3	55.7	0.0	0.0	6.980465	12208	12756	484-1181	1.000000	52.0
trnR-UCU-atpA_p5	trnR-UCU-atpA	5	GGTTCAAATCCTATTGGACGC	GGAACTCTTTTTACTTCAGGAACAA	21	25	47.6	36.0	57.3	57.7	0.0	0.0	7.022379	12208	12756	483-1180	1.000000	52.0
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	31579	32887	1242-1425	1.000000	90.1
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	31579	32887	1303-1486	1.000000	88.5
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	31579	32887	1260-1443	1.000000	87.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.706353	31579	32887	1321-1504	1.000000	86.3
trnC-GCA-petN_p5	trnC-GCA-petN	5	GTATAGTTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	57.2	58.2	14.3	0.0	0.925787	31579	32887	1294-1477	1.000000	84.6
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CCGGTACATCATTATGGATTGGA	GCGTCTTCTCTTTGGCAAAG	23	20	43.5	50.0	58.1	57.7	0.0	29.6	3.416943	41493	42512	679-1602	1.000000	64.7
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TCCGGTACATCATTATGGATTGG	GCGTCTTCTCTTTGGCAAAG	23	20	43.5	50.0	58.1	57.7	0.0	29.6	3.416943	41493	42512	680-1603	1.000000	64.7
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	CCGGTACATCATTATGGATTGG	GCGTCTTCTCTTTGGCAAAG	22	20	45.5	50.0	56.7	57.7	0.0	29.6	3.619721	41493	42512	679-1602	1.000000	63.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CCGGTACATCATTATGGATTGGA	AACCCGCGTCTTCTCTTTG	23	19	43.5	52.6	58.1	58.1	0.0	4.9	4.180192	41493	42512	684-1607	1.000000	58.6
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TCCGGTACATCATTATGGATTGG	AACCCGCGTCTTCTCTTTG	23	19	43.5	52.6	58.1	58.1	0.0	4.9	4.180192	41493	42512	685-1608	1.000000	58.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	52329	54274	1330-2690	1.000000	90.9
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	52329	54274	1338-2698	1.000000	90.4
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	52329	54274	1362-2722	1.000000	90.0
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	52329	54274	1370-2730	1.000000	89.5
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	52329	54274	1344-2704	1.000000	89.0
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.539129	57805	58785	692-1124	1.000000	87.7
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	CCTTTGCTAATCGGGGCTAA	GAAGGTCTACGGTTCGAGTC	20	20	50.0	55.0	57.7	57.5	0.0	0.0	0.882901	57805	58785	691-1123	1.000000	84.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	CTCTGGCCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.7	57.8	0.0	0.0	0.893974	57805	58785	699-1131	1.000000	84.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TCTCTGGCCCTTTGCTAATC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.3	57.8	0.0	0.0	0.914922	57805	58785	700-1132	1.000000	84.7
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCTTTGCTAATCGGGGCTAA	GTTCGAGTCCGTATAGCCCT	20	20	50.0	55.0	57.7	58.7	0.0	0.0	1.025195	57805	58785	680-1112	1.000000	83.8
rbcL-accD_p1	rbcL-accD	1	AATGAGGGACGTGATCTTGC	GTTCCCAAGGCCGACTAAAT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.154115	64075	65244	1334-1850	1.000000	88.2
rbcL-accD_p2	rbcL-accD	2	AATGAGGGACGTGATCTTGC	ATGATCGTAGTCGGGATCGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136294	64075	65244	1541-2057	1.000000	87.9
rbcL-accD_p3	rbcL-accD	3	GCCGTAGCTAATCGAGTAGC	ATGATCGTAGTCGGGATCGT	20	20	55.0	50.0	58.0	58.1	31.7	0.0	0.140034	64075	65244	1586-2102	1.000000	87.7
rbcL-accD_p4	rbcL-accD	4	CGCTGGTACCGTAGTAGGTA	ATGATCGTAGTCGGGATCGT	20	20	55.0	50.0	58.0	58.1	7.6	0.0	0.152897	64075	65244	1854-2370	1.000000	87.0
rbcL-accD_p5	rbcL-accD	5	GAATTGGGCGTTCCTATCGT	ATGATCGTAGTCGGGATCGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.156990	64075	65244	2060-2576	1.000000	86.7
accD-psaI_p1	accD-psaI	1	AGGGCGTTCTGAGTGAGTTA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.4	58.0	0.0	26.9	0.397961	66736	67409	563-1734	1.000000	88.8
accD-psaI_p2	accD-psaI	2	AGGGCGTTCTGAGTGAGTTA	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.4	57.9	0.0	26.8	0.474494	66736	67409	569-1740	1.000000	88.2
accD-psaI_p3	accD-psaI	3	GGGCGTTCTGAGTGAGTTAT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.3	58.0	0.0	26.9	0.701799	66736	67409	562-1733	1.000000	86.4
accD-psaI_p4	accD-psaI	4	TAAAGGGCGTTCTGAGTGAG	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.3	58.0	0.0	26.9	0.770639	66736	67409	566-1737	1.000000	85.8
accD-psaI_p5	accD-psaI	5	GGGCGTTCTGAGTGAGTTAT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.3	57.9	0.0	26.8	0.778333	66736	67409	568-1739	1.000000	85.8
petA-psbJ_p1	petA-psbJ	1	TGAGAAGGTTCAATTGGCTGA	ATTCCGGATTGGGTTCATCC	21	20	42.9	50.0	57.5	57.6	5.4	4.1	1.872134	70578	71936	852-1860	1.000000	73.6
petA-psbJ_p2	petA-psbJ	2	TGAGAAGGTTCAATTGGCTG	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	55.9	57.6	5.4	4.1	2.427149	70578	71936	852-1860	1.000000	69.1
petA-psbJ_p3	petA-psbJ	3	TGAGAAGGTTCAATTGGCTGAA	ATTCCGGATTGGGTTCATCC	22	20	40.9	50.0	58.2	57.6	5.4	4.1	2.533649	70578	71936	852-1860	1.000000	68.3
petA-psbJ_p4	petA-psbJ	4	TTGAGAAGGTTCAATTGGCTGA	ATTCCGGATTGGGTTCATCC	22	20	40.9	50.0	58.2	57.6	5.4	4.1	2.533649	70578	71936	853-1861	1.000000	68.3
petA-psbJ_p5	petA-psbJ	5	TTGAGAAGGTTCAATTGGCTG	ATTCCGGATTGGGTTCATCC	21	20	42.9	50.0	56.7	57.6	5.4	4.1	2.656059	70578	71936	853-1861	1.000000	67.3
rpl33-rps18_p1	rpl33-rps18	1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.390844	76335	76645	413-647	1.000000	86.6
rpl33-rps18_p2	rpl33-rps18	2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.9	57.7	0.0	0.0	0.390844	76335	76645	414-648	1.000000	86.6
rpl33-rps18_p3	rpl33-rps18	3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	20	20	45.0	50.0	57.6	57.7	0.0	0.0	0.617852	76335	76645	415-649	1.000000	84.8
rpl33-rps18_p4	rpl33-rps18	4	TCTGTCCCTGTTGTTGCAAA	ATTCGATCCCCCGATTGGAT	20	20	45.0	50.0	57.9	58.6	0.0	0.0	0.774074	76335	76645	410-644	1.000000	83.6
rpl33-rps18_p5	rpl33-rps18	5	TTCTGTCCCTGTTGTTGCAA	ATTCGATCCCCCGATTGGAT	20	20	45.0	50.0	57.9	58.6	0.0	0.0	0.774074	76335	76645	411-645	1.000000	83.5
rps11-rpl36_p1	rps11-rpl36	1	TACGCGCATTCTTACGTGAA	CGTAGGCGTGGACGAATTAG	20	20	45.0	55.0	58.0	58.5	5.7	2.7	0.546086	88087	88483	487-592	1.000000	87.6
rps11-rpl36_p2	rps11-rpl36	2	TTACGCGCATTCTTACGTGA	CGTAGGCGTGGACGAATTAG	20	20	45.0	55.0	58.0	58.5	5.7	2.7	0.546086	88087	88483	488-593	1.000000	87.6
rps11-rpl36_p3	rps11-rpl36	3	CCCTTTGGTATTTTACGCGC	CGTAGGCGTGGACGAATTAG	20	20	50.0	55.0	57.8	58.5	5.7	2.7	0.708887	88087	88483	500-605	1.000000	86.3
rps11-rpl36_p4	rps11-rpl36	4	TACGCGCATTCTTACGTGAA	AGGCGTGGACGAATTAGAGT	20	20	45.0	50.0	58.0	58.8	5.7	2.7	0.836554	88087	88483	484-589	1.000000	85.3
rps11-rpl36_p5	rps11-rpl36	5	TTACGCGCATTCTTACGTGA	AGGCGTGGACGAATTAGAGT	20	20	45.0	50.0	58.0	58.8	5.7	2.7	0.836554	88087	88483	485-590	1.000000	85.3
rps8-rpl14_p1	rps8-rpl14	1	AGCAATGGTGTCTTTACCCA	TCGAATTTTTGGTGCGATCG	20	20	45.0	45.0	57.0	57.5	0.0	11.7	1.488824	89491	89958	526-973	1.000000	80.1
rps8-rpl14_p2	rps8-rpl14	2	AGCAATGGTGTCTTTACCCA	GAATTTTTGGTGCGATCGCC	20	20	45.0	50.0	57.0	58.7	0.0	35.1	1.701556	89491	89958	524-971	1.000000	78.4
rps8-rpl14_p3	rps8-rpl14	3	TCAGCAATGGTGTCTTTACCC	TCGAATTTTTGGTGCGATCG	21	20	47.6	45.0	58.2	57.5	12.4	11.7	1.728569	89491	89958	528-975	1.000000	78.2
rps8-rpl14_p4	rps8-rpl14	4	CAGCAATGGTGTCTTTACCC	TCGAATTTTTGGTGCGATCG	20	20	50.0	45.0	56.7	57.5	4.0	11.7	1.822479	89491	89958	527-974	1.000000	77.4
rps8-rpl14_p5	rps8-rpl14	5	TCAGCAATGGTGTCTTTACCC	GAATTTTTGGTGCGATCGCC	21	20	47.6	50.0	58.2	58.7	12.4	35.1	1.941302	89491	89958	526-973	1.000000	76.5
rps3-rps19_p1	rps3-rps19	1	TACCAAGTCTGAAACCGAGC	ACTCTAAACTTCCGGGGACA	20	20	50.0	50.0	57.5	58.0	0.0	0.0	0.470417	92786	93414	631-811	1.000000	85.1
rps3-rps19_p2	rps3-rps19	2	GTCTGAAACCGAGCGGATTT	ACTCTAAACTTCCGGGGACA	20	20	50.0	50.0	58.6	58.0	16.8	0.0	0.580129	92786	93414	625-805	1.000000	84.3
rps3-rps19_p3	rps3-rps19	3	TGGTTGTGCAAACCAAAGAG	ACTCTAAACTTCCGGGGACA	20	20	45.0	50.0	57.0	58.0	22.9	0.0	0.977298	92786	93414	669-849	1.000000	80.8
rps3-rps19_p4	rps3-rps19	4	TCTGAAACCGAGCGGATTTA	ACTCTAAACTTCCGGGGACA	20	20	45.0	50.0	57.0	58.0	16.8	0.0	1.062381	92786	93414	624-804	1.000000	80.4
rps3-rps19_p5	rps3-rps19	5	TACCAAGTCTGAAACCGAGC	CTAAACTTCCGGGGACATGC	20	20	50.0	55.0	57.5	58.6	0.0	0.0	1.071083	92786	93414	628-808	1.000000	80.3
ndhF-rpl32_p1	ndhF-rpl32	1	CCGTGGGAAAAAGGAGAAGT	TCAATGCTGCCCAATATCCC	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.272296	121811	122771	1133-1169	1.000000	82.9
ndhF-rpl32_p2	ndhF-rpl32	2	CCGTGGGAAAAAGGAGAAGT	CAATGCTGCCCAATATCCCT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.277314	121811	122771	1132-1168	1.000000	82.9
ndhF-rpl32_p3	ndhF-rpl32	3	TTTGTTGCCGTGGGAAAAAG	TCAATGCTGCCCAATATCCC	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.301929	121811	122771	1140-1176	1.000000	82.6
ndhF-rpl32_p4	ndhF-rpl32	4	TTTGTTGCCGTGGGAAAAAG	CAATGCTGCCCAATATCCCT	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.306947	121811	122771	1139-1175	1.000000	82.6
ndhF-rpl32_p5	ndhF-rpl32	5	CCGTGGGAAAAAGGAGAAGT	AAAAGCTTTCAATGCTGCCC	20	20	50.0	45.0	57.7	57.8	0.0	13.1	0.446307	121811	122771	1141-1177	1.000000	81.5
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGATATTGGGCAGCATTGA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.008182	122940	123744	897-1013	1.000000	87.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGATATTGGGCAGCATTGA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.009553	122940	123744	898-1014	1.000000	87.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGATATTGGGCAGCATTG	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.013201	122940	123744	898-1014	1.000000	87.3
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGATATTGGGCAGCATTG	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.014571	122940	123744	899-1015	1.000000	87.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGATATTGGGCAGCATTGA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.124629	122940	123744	907-1023	1.000000	86.3
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCTGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	20	20	45.0	45.0	55.4	57.9	0.0	0.0	2.658418	128434	128967	565-666	1.000000	70.7
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCTGATCCCAGAAAGA	TTTGGGCCGTTTACCAATGT	20	20	45.0	45.0	55.4	58.3	0.0	0.0	2.854865	128434	128967	554-655	1.000000	69.2
ndhG-ndhI_p3	ndhG-ndhI	3	AGACCTGATCCCAGAAAGAT	AATCAAATTGCTTTGGGCCG	20	20	45.0	45.0	55.2	57.9	0.0	0.0	2.900528	128434	128967	564-665	1.000000	68.8
ndhG-ndhI_p4	ndhG-ndhI	4	AAGACCTGATCCCAGAAAGA	CTTTGGGCCGTTTACCAATG	20	20	45.0	50.0	55.4	57.6	0.0	0.0	2.922907	128434	128967	555-656	1.000000	68.6
ndhG-ndhI_p5	ndhG-ndhI	5	AAGACCTGATCCCAGAAAGA	CAAATTGCTTTGGGCCGTTT	20	20	45.0	45.0	55.4	58.4	0.0	0.0	2.968674	128434	128967	562-663	1.000000	68.3