Hotspot

ycf1

SSC · 121495-127041 · 5547 bp

Back to Glechoma

Pi 0.00382

MarkerSeek score 41.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5547
variable_sites: 41
pi: 0.00382
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGCTAATTTTTCGACCATCAGG	TGGTATACTTCTTTCAGATGCTGA	40.9	37.5	57.0	57.5	239-260 bp	1.0	46.5
ycf1_p2	CTGCTAATTTTTCGACCATCAGG	TGGTATACTTCTTTCAGATGCTGA	43.5	37.5	58.1	57.5	240-261 bp	1.0	46.5
ycf1_p3	TGCTAATTTTTCGACCATCAGG	TGGTATACTTCTTTCAGATGCTG	40.9	39.1	57.0	56.1	239-260 bp	1.0	46.5
ycf1_p4	GCTAATTTTTCGACCATCAGGA	TGGTATACTTCTTTCAGATGCTGA	40.9	37.5	56.8	57.5	259 bp	0.75	30.9
ycf1_p5	TGCTAATTTTTCGACCATCAGGA	TGGTATACTTCTTTCAGATGCTGA	39.1	37.5	58.4	57.5	260 bp	0.75	30.9