Hotspot

rpoC1

LSC · 20513-23331 · 2819 bp

Back to Glechoma

Pi 0.00272

MarkerSeek score 45.20

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2819
variable_sites: 15
pi: 0.00272
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCGGTCTTGATTCCCGTAGA	GGAATAAATCCGAATTTGTATTCT	50.0	29.2	57.9	53.5	2944-2948 bp	1.0	40.4
rpoC1_p2	TTTTCGGTCTTGATTCCCGT	GGAATAAATCCGAATTTGTATTCT	45.0	29.2	57.4	53.5	2947-2951 bp	1.0	40.4
rpoC1_p3	TCGGTCTTGATTCCCGTAGA	GGAATAAATCCGAATTTGTATTC	50.0	30.4	57.9	52.0	2944-2948 bp	1.0	40.4
rpoC1_p4	TCCCGTAGAAATTCCGGAAC	GGAATAAATCCGAATTTGTATTCT	50.0	29.2	57.3	53.5	2933-2937 bp	1.0	40.4
rpoC1_p5	CCCGTAGAAATTCCGGAACT	GGAATAAATCCGAATTTGTATTCT	50.0	29.2	57.3	53.5	2932-2936 bp	1.0	40.4