Hotspot

petB

LSC · 73954-75318 · 1365 bp

Back to Glechoma

Pi 0.00147

MarkerSeek score 60.50

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1365
variable_sites: 4
pi: 0.00147
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	AAGGATCACAAAGTCCTGGC	TCCTATCATTCCTCCTCTAAGTGA	50.0	41.7	57.8	57.7	1541-1546 bp	1.0	44.7
petB_p2	GGATCACAAAGTCCTGGCTT	TCCTATCATTCCTCCTCTAAGTGA	50.0	41.7	57.8	57.7	1539-1544 bp	1.0	44.7
petB_p3	AAGGATCACAAAGTCCTGGC	CCTATCATTCCTCCTCTAAGTGA	50.0	43.5	57.8	56.3	1540-1545 bp	1.0	41.7
petB_p4	GGATCACAAAGTCCTGGCTT	CCTATCATTCCTCCTCTAAGTGA	50.0	43.5	57.8	56.3	1538-1543 bp	1.0	41.7
petB_p5	GGATCACAAAGTCCTGGCTT	TCCTATCATTCCTCCTCTAAGTG	50.0	43.5	57.8	56.3	1539-1544 bp	1.0	41.7