Hotspot

ndhF

SSC · 108545-110758 · 2214 bp

Back to Glechoma

Pi 0.00339

MarkerSeek score 50.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2214
variable_sites: 15
pi: 0.00339
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGACCCGTTTCTGAAGAGTC	TGACTCCTTTCAAAAGAAGT	50.0	35.0	56.9	52.2	2356-2360 bp	1.0	40.5
ndhF_p2	GACCCGTTTCTGAAGAGTCT	TGACTCCTTTCAAAAGAAGT	50.0	35.0	56.9	52.2	2355-2359 bp	1.0	40.5
ndhF_p3	AGACCCGTTTCTGAAGAGTC	TCCTTTCAAAAGAAGTAAAATCCGA	50.0	32.0	56.9	57.2	2352-2356 bp	1.0	40.5
ndhF_p4	GACCCGTTTCTGAAGAGTCT	TCCTTTCAAAAGAAGTAAAATCCGA	50.0	32.0	56.9	57.2	2351-2355 bp	1.0	40.5
ndhF_p5	GACCCGTTTCTGAAGAGTCT	TTGACTCCTTTCAAAAGAAGT	50.0	33.3	56.9	53.2	2356-2360 bp	1.0	40.5