Hotspot

clpP1

LSC · 69117-71050 · 1934 bp

Back to Glechoma

Pi 0.00181

MarkerSeek score 59.40

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 1934
variable_sites: 7
pi: 0.00181
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TGGGCTGGTTTAGATTGATCC	ACGTTTCCAAATCAAAGTGA	47.6	35.0	57.7	53.6	2086-2092 bp	1.0	40.5
clpP1_p2	TGGGCTGGTTTAGATTGATCC	TGTTACGTTTCCAAATCAAAGTGA	47.6	33.3	57.7	57.6	2090-2096 bp	1.0	40.5
clpP1_p3	TGGGCTGGTTTAGATTGATCC	ACGTTTCCAAATCAAAGTGAA	47.6	33.3	57.7	54.5	2086-2092 bp	1.0	40.5
clpP1_p4	GGGCTGGTTTAGATTGATCCT	ACGTTTCCAAATCAAAGTGA	47.6	35.0	57.4	53.6	2085-2091 bp	1.0	40.5
clpP1_p5	TGGGCTGGTTTAGATTGATCC	ACGTTTCCAAATCAAAGTGAAA	47.6	31.8	57.7	55.3	2086-2092 bp	1.0	40.5