Hotspot

ccsA

SSC · 112315-113292 · 978 bp

Back to Glechoma

Pi 0.00409

MarkerSeek score 48.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 8
pi: 0.00409
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	CGCGAATCAAAGTAACGACG	50.0	50.0	57.9	58.0	1269 bp	0.75	69.3
ccsA_p2	ACAAGCCGCTATGGTGAAAT	CGCGAATCAAAGTAACGACG	45.0	50.0	57.9	58.0	1292 bp	0.75	69.3
ccsA_p3	AAATTGGTAGACACGCTGCT	CGCGAATCAAAGTAACGACG	45.0	50.0	57.8	58.0	1276 bp	0.75	68.8
ccsA_p4	GGTAGACACGCTGCTCTTAG	CGCGAATCAAAGTAACGACG	55.0	50.0	57.8	58.0	1271 bp	0.75	68.7
ccsA_p5	GTAGACACGCTGCTCTTAGG	CGCGAATCAAAGTAACGACG	55.0	50.0	57.8	58.0	1270 bp	0.75	68.7