Hotspot

rpl20-rps12

LSC · 68082-68879 · 798 bp

Back to Glechoma

Pi 0.00126
MarkerSeek score 60.10
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
798
variable_sites
2
pi
0.00126
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TCCGAGCTATATATCCGCGT ACAGCCCATTCGAAATGTCA 50.0 45.0 58.0 57.8 907-911 bp 1.0 78.7
rpl20-rps12_p2 TCCGAGCTATATATCCGCGT ACAAGACAGCCCATTCGAAA 50.0 45.0 58.0 57.7 912-916 bp 1.0 78.1
rpl20-rps12_p3 ACCCTCGAAAACTTGATGCA ACAGCCCATTCGAAATGTCA 45.0 45.0 57.7 57.8 952-956 bp 1.0 76.7
rpl20-rps12_p4 TCCGAGCTATATATCCGCGT AGACAGCCCATTCGAAATGT 50.0 45.0 58.0 57.5 909-913 bp 1.0 76.3
rpl20-rps12_p5 ACCCTCGAAAACTTGATGCA ACAAGACAGCCCATTCGAAA 45.0 45.0 57.7 57.7 957-961 bp 1.0 76.2