Hotspot

petN-psbM

LSC · 28043-29004 · 962 bp

Back to Glechoma

Pi 0.00885

MarkerSeek score 65.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 962
variable_sites: 15
pi: 0.00885
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	1047-1051 bp	1.0	60.5
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	AGTTCCTACCGCTTTTCTACT	50.0	42.9	58.0	56.4	1045-1049 bp	1.0	60.5
petN-psbM_p3	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.0	55.4	1054-1058 bp	1.0	60.4
petN-psbM_p4	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACCGCT	50.0	45.0	58.0	55.4	1052-1056 bp	1.0	60.4
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	AGTTCCTACCGCTTTTCTACT	55.0	42.9	57.9	56.4	1044-1048 bp	1.0	59.9