Hotspot

trnS-GCU-trnG-UCC

LSC · 8025-8742 · 718 bp

Back to Glechoma

Pi 0.00627

MarkerSeek score 58.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 718
variable_sites: 8
pi: 0.00627
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	755-773 bp	1.0	63.8
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACCACTAAACTATACCCGCT	50.0	45.0	58.0	55.6	754-772 bp	1.0	63.8
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	ACCACTAAACTATACCCGCT	50.0	45.0	58.2	55.6	809-827 bp	1.0	62.5
trnS-GCU-trnG-UCC_p4	AGCAATCCGACGCTTTAGTC	ACCACTAAACTATACCCGCT	50.0	45.0	58.4	55.6	757-775 bp	1.0	61.0
trnS-GCU-trnG-UCC_p5	ACCAACGGAAAGAGAGGGAT	ACCACTAAACTATACCCGCT	50.0	45.0	58.3	55.6	813-831 bp	1.0	60.9