Hotspot

trnH-GUG-psbA

LSC · 79-423 · 345 bp

Back to Glechoma

Pi 0.0275
MarkerSeek score 74.60
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
345
variable_sites
15
pi
0.0275
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.93
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CTAGACCTAGCCGCTATCGA 55.0 55.0 58.1 57.9 370-405 bp 1.0 85.3
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG TCTAGACCTAGCCGCTATCG 55.0 55.0 58.1 57.9 371-406 bp 1.0 85.3
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA CTAGACCTAGCCGCTATCGA 50.0 55.0 57.9 57.9 384-419 bp 1.0 85.2
trnH-GUG-psbA_p4 CAATCCACTGCCTTGATCCA TCTAGACCTAGCCGCTATCG 50.0 55.0 57.9 57.9 385-420 bp 1.0 85.2
trnH-GUG-psbA_p5 AATCCACTGCCTTGATCCAC CTAGACCTAGCCGCTATCGA 50.0 55.0 57.9 57.9 383-418 bp 1.0 85.2