Hotspot

rps15

SSC · 120247-120519 · 273 bp

Back to Gladiolus

Pi 0.536

MarkerSeek score 77.10

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 140
pi: 0.536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	CCTCTCCACCAATAATGCCC	TCCCAGACGTCGTAAAGAGT	55.0	50.0	58.0	58.1	1765 bp	0.5	59.1
rps15_p2	TTTCCAATCGTTCTGGTGCA	TCCCAGACGTCGTAAAGAGT	45.0	50.0	58.0	58.1	1399 bp	0.5	59.0
rps15_p3	GCCATAAAAGGACCCAACCA	TCCCAGACGTCGTAAAGAGT	50.0	50.0	58.1	58.1	1496 bp	0.5	58.7
rps15_p4	TGCCATAAAAGGACCCAACC	TCCCAGACGTCGTAAAGAGT	50.0	50.0	58.1	58.1	1497 bp	0.5	58.7
rps15_p5	GGCGGCTTCGAATAGATCAT	TCCCAGACGTCGTAAAGAGT	50.0	50.0	57.9	58.1	1572 bp	0.5	58.5