Hotspot

ndhI

SSC · 116150-116689 · 540 bp

Back to Gladiolus

Pi 0.457

MarkerSeek score 80.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 540
variable_sites: 220
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGTAATACTACCCCCAGCCC	CGTATGAAGCCGAGGTTTCA	55.0	50.0	57.9	58.0	1572 bp	0.5	59.1
ndhI_p2	GTAATACTACCCCCAGCCCT	CGTATGAAGCCGAGGTTTCA	55.0	50.0	57.9	58.0	1571 bp	0.5	59.1
ndhI_p3	AGTAATACTACCCCCAGCCC	TACCCGAAGCAGAAGAGGAA	55.0	50.0	57.9	58.1	1356 bp	0.5	58.6
ndhI_p4	GTAATACTACCCCCAGCCCT	TACCCGAAGCAGAAGAGGAA	55.0	50.0	57.9	58.1	1355 bp	0.5	58.6
ndhI_p5	GTAATACTACCCCCAGCCCT	TTACCCGAAGCAGAAGAGGA	55.0	50.0	57.9	58.1	1356 bp	0.5	58.6