Hotspot

ndhH

SSC · 118958-120139 · 1182 bp

Back to Gladiolus

Pi 0.473

MarkerSeek score 81.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 481
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CCAACGGACCAGCATATTCA	TATCGCAAAGGGGTCTACGA	50.0	50.0	58.0	58.2	1632 bp	0.5	57.8
ndhH_p2	AAGTAGTTTTGCCCCGTCTG	TATCGCAAAGGGGTCTACGA	50.0	50.0	58.1	58.2	1670 bp	0.5	57.3
ndhH_p3	CGGACCAGCATATTCAGGAC	TATCGCAAAGGGGTCTACGA	55.0	50.0	58.1	58.2	1628 bp	0.5	57.1
ndhH_p4	GGGCAATACTTGAAATGGCG	TATCGCAAAGGGGTCTACGA	50.0	50.0	57.8	58.2	1845 bp	0.5	56.5
ndhH_p5	GGACCAGCATATTCAGGACC	TATCGCAAAGGGGTCTACGA	55.0	50.0	57.7	58.2	1627 bp	0.5	56.0