Hotspot

ccsA

SSC · 111500-112456 · 957 bp

Back to Gladiolus

Pi 0.464

MarkerSeek score 83.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 957
variable_sites: 413
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	CTCAAAAACTCGCACGAACC	50.0	50.0	58.0	58.0	1183 bp	0.5	59.8
ccsA_p2	GTGAAATCGGTAGACACGCT	GGTTCTCAAAAACTCGCACG	50.0	50.0	58.0	58.0	1187 bp	0.5	59.8
ccsA_p3	TGAAATCGGTAGACACGCTG	CTCAAAAACTCGCACGAACC	50.0	50.0	58.0	58.0	1182 bp	0.5	59.8
ccsA_p4	TGAAATCGGTAGACACGCTG	GGTTCTCAAAAACTCGCACG	50.0	50.0	58.0	58.0	1186 bp	0.5	59.8
ccsA_p5	GTGAAATCGGTAGACACGCT	TGCATTACCGGGAATGAGTG	50.0	50.0	58.0	58.0	1691 bp	0.5	59.6