Hotspot

rps15-ycf1

SSC · 120520-120975 · 456 bp

Back to Gladiolus

Pi 0.435

MarkerSeek score 77.10

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 456
variable_sites: 164
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TTTCCAATCGTTCTGGTGCA	TCCCAGACGTCGTAAAGAGT	45.0	50.0	58.0	58.1	1399 bp	0.5	59.0
rps15-ycf1_p2	GCCATAAAAGGACCCAACCA	TCCCAGACGTCGTAAAGAGT	50.0	50.0	58.1	58.1	1496 bp	0.5	58.7
rps15-ycf1_p3	TGCCATAAAAGGACCCAACC	TCCCAGACGTCGTAAAGAGT	50.0	50.0	58.1	58.1	1497 bp	0.5	58.7
rps15-ycf1_p4	TTTCCAATCGTTCTGGTGCA	TCGAACCAATCCGTCTCTCT	45.0	50.0	58.0	58.2	1601 bp	0.5	58.5
rps15-ycf1_p5	TTTCCAATCGTTCTGGTGCA	AAAGAATAAGCACCACGGGG	45.0	50.0	58.0	58.2	1525 bp	0.5	58.5